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20th anniversary collection

New Content Item2022 marks an exciting milestone for . We celebrate 20 years of publishing research and timely reviews on the use and investigation of microbial cells as producers of recombinant proteins and natural products, or as catalyzers of biological transformations of industrial interest. Conceived by Founding Editor-in-Chief, Prof Antonio Villaverde (Universitat Autònoma de Barcelona, Spain) and launched as a fully open access journal by ¾Á¸»ŠÊ˜·³Ç in 2002, Microbial Cell Factories has grown to become the world leading, primary research journal fully focusing on applied microbiology. In this 20th anniversary collection, we present the top twenty most cited publications in the journal (all time, as of January 2022) that demonstrate the lasting impact of the journal on developments in the field.


  1. Microalgae have recently attracted considerable interest worldwide, due to their extensive application potential in the renewable energy, biopharmaceutical, and nutraceutical industries. Microalgae are renewab...

    Authors: Muhammad Imran Khan, Jin Hyuk Shin and Jong Deog Kim
    Citation: Microbial Cell Factories 2018 17:36
  2. The aim of this review is to summarize the effect in host energy metabolism of the production of B group vitamins and short chain fatty acids (SCFA) by commensal, food-grade and probiotic bacteria, which are a...

    Authors: Jean Guy LeBlanc, Florian Chain, Rebeca Martín, Luis G. Bermúdez-Humarán, Stéphanie Courau and Philippe Langella
    Citation: Microbial Cell Factories 2017 16:79
  3. Isolated from a wide range of sources, the genus Paenibacillus comprises bacterial species relevant to humans, animals, plants, and the environment. Many Paenibacillus species can promote crop growth directly via...

    Authors: Elliot Nicholas Grady, Jacqueline MacDonald, Linda Liu, Alex Richman and Ze-Chun Yuan
    Citation: Microbial Cell Factories 2016 15:203
  4. Anaerobic digestion has been widely applied to treat the waste activated sludge from biological wastewater treatment and produce methane for biofuel, which has been one of the most efficient solutions to both ...

    Authors: Jianhua Guo, Yongzhen Peng, Bing-Jie Ni, Xiaoyu Han, Lu Fan and Zhiguo Yuan
    Citation: Microbial Cell Factories 2015 14:33
  5. Bacteriocins are heat-stable ribosomally synthesized antimicrobial peptides produced by various bacteria, including food-grade lactic acid bacteria (LAB). These antimicrobial peptides have huge potential as bo...

    Authors: Rodney H Perez, Takeshi Zendo and Kenji Sonomoto
    Citation: Microbial Cell Factories 2014 13(Suppl 1):S3

    This article is part of a Supplement:

  6. Current soil management strategies are mainly dependent on inorganic chemical-based fertilizers, which caused a serious threat to human health and environment. The exploitation of beneficial microbes as a biof...

    Authors: Deepak Bhardwaj, Mohammad Wahid Ansari, Ranjan Kumar Sahoo and Narendra Tuteja
    Citation: Microbial Cell Factories 2014 13:66
  7. Adaptive laboratory evolution is a frequent method in biological studies to gain insights into the basic mechanisms of molecular evolution and adaptive changes that accumulate in microbial populations during l...

    Authors: Martin Dragosits and Diethard Mattanovich
    Citation: Microbial Cell Factories 2013 12:64
  8. Omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provide significant health benefits and this has led to an increased consumption as dietary supplements. Omega-3 fatty acids EPA a...

    Authors: T Catalina Adarme-Vega, David K Y Lim, Matthew Timmins, Felicitas Vernen, Yan Li and Peer M Schenk
    Citation: Microbial Cell Factories 2012 11:96
  9. Production of correctly disulfide bonded proteins to high yields remains a challenge. Recombinant protein expression in Escherichia coli is the popular choice, especially within the research community. While ther...

    Authors: Julie Lobstein, Charlie A Emrich, Chris Jeans, Melinda Faulkner, Paul Riggs and Mehmet Berkmen
    Citation: Microbial Cell Factories 2012 11:753

    The has been published in Microbial Cell Factories 2016 15:124

  10. Bacteria are simple and cost effective hosts for producing recombinant proteins. However, their physiological features may limit their use for obtaining in native form proteins of some specific structural clas...

    Authors: Ario de Marco
    Citation: Microbial Cell Factories 2009 8:26
  11. Most of the hosts used to produce the 151 recombinant pharmaceuticals so far approved for human use by the Food and Drug Administration (FDA) and/or by the European Medicines Agency (EMEA) are microbial cells,...

    Authors: Neus Ferrer-Miralles, Joan Domingo-Espín, José Luis Corchero, Esther Vázquez and Antonio Villaverde
    Citation: Microbial Cell Factories 2009 8:17
  12. Increasing energy costs and environmental concerns have motivated engineering microbes for the production of "second generation" biofuels that have better properties than ethanol.

    Authors: Eric J Steen, Rossana Chan, Nilu Prasad, Samuel Myers, Christopher J Petzold, Alyssa Redding, Mario Ouellet and Jay D Keasling
    Citation: Microbial Cell Factories 2008 7:36
  13. Laccases are multi-copper containing oxidases (EC 1.10.3.2), widely distributed in fungi, higher plants and bacteria. Laccase catalyses the oxidation of phenols, polyphenols and anilines by one-electron abstra...

    Authors: Adinarayana Kunamneni, Susana Camarero, Carlos García-Burgos, Francisco J Plou, Antonio Ballesteros and Miguel Alcalde
    Citation: Microbial Cell Factories 2008 7:32
  14. Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or indu...

    Authors: Brigitte Gasser, Markku Saloheimo, Ursula Rinas, Martin Dragosits, Escarlata Rodríguez-Carmona, Kristin Baumann, Maria Giuliani, Ermenegilda Parrilli, Paola Branduardi, Christine Lang, Danilo Porro, Pau Ferrer, Maria Luisa Tutino, Diethard Mattanovich and Antonio Villaverde
    Citation: Microbial Cell Factories 2008 7:11
  15. In today's world, there is an increasing trend towards the use of renewable, cheap and readily available biomass in the production of a wide variety of fine and bulk chemicals in different biorefineries. Biore...

    Authors: Pernilla Turner, Gashaw Mamo and Eva Nordberg Karlsson
    Citation: Microbial Cell Factories 2007 6:9
  16. The methylotrophic yeast Pichia pastoris has been widely reported as a suitable expression system for heterologous protein production. The use of different phenotypes under PAOX promoter, other alternative promot...

    Authors: Oriol Cos, Ramón Ramón, José Luis Montesinos and Francisco Valero
    Citation: Microbial Cell Factories 2006 5:17
  17. This article describes the use of biofilm reactors for the production of various chemicals by fermentation and wastewater treatment. Biofilm formation is a natural process where microbial cells attach to the s...

    Authors: Nasib Qureshi, Bassam A Annous, Thaddeus C Ezeji, Patrick Karcher and Ian S Maddox
    Citation: Microbial Cell Factories 2005 4:24
  18. Microorganisms are used in large-scale heap or tank aeration processes for the commercial extraction of a variety of metals from their ores or concentrates. These include copper, cobalt, gold and, in the past,...

    Authors: Douglas E Rawlings
    Citation: Microbial Cell Factories 2005 4:13
  19. Pure, soluble and functional proteins are of high demand in modern biotechnology. Natural protein sources rarely meet the requirements for quantity, ease of isolation or price and hence recombinant technology ...

    Authors: Hans Peter Sørensen and Kim Kusk Mortensen
    Citation: Microbial Cell Factories 2005 4:1
  20. Recent advances in generating active proteins through refolding of bacterial inclusion body proteins are summarized in conjunction with a short overview on inclusion body isolation and solubilization procedure...

    Authors: Luis Felipe Vallejo and Ursula Rinas
    Citation: Microbial Cell Factories 2004 3:11