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CRISPR/Cas9 and the microbial cell factory

CRISPR/Cas9, including the derived CRISPRi, has evolved quickly into a popular technology to edit the genomes of prokaryotes and eukaryotes. For constructing microbial cell factories (MCF), it is especially important to regulate metabolic flux toward the target products. To achieve maximal flux channelling toward target products, the regulation of expression of multiple genes is desirable, which is labor intensive and time consuming in most cases. CRISPR/Cas9, especially CRISPRi, allows the design of multiple single strange-guided RNA (sg RNA). These bind to the target genes with different repression intensities with the help of dCas9, and thus regulate metabolic flux. The technology provides a convenient tool to enhance production of various chemicals, regulating cell morphology and changing growth pattern. It is a technology that will revolutionize the metabolic engineering of microbial cells.

  1. Corynebacterium glutamicum (C. glutamicum) has traditionally been used as a microbial cell factory for the industrial production of many amino acids and other industrially important co...

    Authors: Feng Peng, Xinyue Wang, Yang Sun, Guibin Dong, Yankun Yang, Xiuxia Liu and Zhonghu Bai
    Citation: Microbial Cell Factories 2017 16:201
    Content type: Research Published on:

  2. Clustered regularly interspaced short palindromic repeats interference (CRISPRi) has provided an efficient approach for targeted gene inhibition. A non-model microorganism Halomonas species TD01 has been develope...

    Authors: Wei Tao, Li Lv and Guo-Qiang Chen
    Citation: Microbial Cell Factories 2017 16:48
    Content type: Research Published on:

  3. Transcriptional reprogramming is a fundamental process of living cells in order to adapt to environmental and endogenous cues. In order to allow flexible and timely control over gene expression without the int...

    Authors: Emil D. Jensen, Raphael Ferreira, Tadas Jako膷i奴nas, Dushica Arsovska, Jie Zhang, Ling Ding, Justin D. Smith, Florian David, Jens Nielsen, Michael K. Jensen and Jay D. Keasling
    Citation: Microbial Cell Factories 2017 16:46
    Content type: Research Published on:

  4. Anthocyanins are a class of brightly colored, glycosylated flavonoid pigments that imbue their flower and fruit host tissues with hues of predominantly red, orange, purple, and blue. Although all anthocyanins ...

    Authors: Brady F. Cress, Quentin D. Leitz, Daniel C. Kim, Teresita D. Amore, Jon Y. Suzuki, Robert J. Linhardt and Mattheos A. G. Koffas
    Citation: Microbial Cell Factories 2017 16:10
    Content type: Research Published on:

  5. Cyanobacterium Synechococcus elongatus PCC 7942 holds promise for biochemical conversion, but gene deletion in PCC 7942 is time-consuming and may be lethal to cells. CRISPR interference (CRISPRi) is an emerging t...

    Authors: Chun-Hung Huang, Claire R. Shen, Hung Li, Li-Yu Sung, Meng-Ying Wu and Yu-Chen Hu
    Citation: Microbial Cell Factories 2016 15:196
    Content type: Research Published on:

  6. Clustered regularly interspaced short palindromic repeats interference (CRISPRi) is a recently developed powerful tool for gene regulation. In Escherichia coli, the type I CRISPR system expressed endogenously sha...

    Authors: Yizhao Chang, Tianyuan Su, Qingsheng Qi and Quanfeng Liang
    Citation: Microbial Cell Factories 2016 15:195
    Content type: Research Published on:

  7. As autotrophic prokaryotes, cyanobacteria are ideal chassis organisms for sustainable production of various useful compounds. The newly characterized cyanobacterium Synechococcus elongatus UTEX 2973 is a promisin...

    Authors: Kristen E. Wendt, Justin Ungerer, Ryan E. Cobb, Huimin Zhao and Himadri B. Pakrasi
    Citation: Microbial Cell Factories 2016 15:115
    Content type: Research Published on: